Impact Factor 3.180
Gene Array Database
Gene arrays of normal human melanocytes
Normal human melanocyte array 1 (Zip file - 5MB download Winzip here)
Normal human melanocyte array 1 (download the MS Excel file - 20MB)
Normal human melanocyte array 2 (Zip file - 5MB download Winzip here)
Normal human melanocyte array 2 (download the MS Excel file - 17MB)
Early passage normal human melanocytes from newborn Caucasian foreskins were grown in Ham's F-12 medium plus 7% fetal bovine serum enriched with 85 nM TPA, 0.1 mM IBMX, 2.5 nM cholera toxin, 1 uM Na3VO4, and 0.1 mM N6,2'-O-dibutyryladenosine 3:5-cyclic monophosphate. Approximately 20-30 million cells were used for total mRNA extraction and subsequent polyA purification per hybridization.
The human Affymetrix GeneChip system HG-U133A microarray was used and contains 11 pairs of match/mismatch 25-m34 oligonucleotide probes for each of ~14,500 transcripts of known genes.
The total HG-U133A signal was normalized to an arbitrary signal intensity value of 500 using MAS 5.0 software.
For full methodology see:
Hoek K, Rimm DL, Williams KR, Zhao H, Ariyan S, Lin A, Kluger HM, Berger AJ, Cheng E, Trombetta ES, Wu T, Niinobe M, Yoshikawa K, Hannigan GE, Halaban R. (2004) Expression profiling reveals novel pathways in the transformation of melanocytes to melanomas. Cancer Res: 64, 5270-5282
Skin layer gene expression profiles
Supplementary material published to:
Skin layer-specific transcriptional profiles in normal and recessive yellow (Mc1re/Mc1re) mice, by Craig S April and Gregory S. Barsh in Pigment Cell Research 19 (3), 194-205.
Figure S1. Unsupervised hierarchical clustering of gene expression patterns in neonatal mouse skin fractions.
(Download the .jpeg file here - 918KB)
Figure S2. Relative expression levels of Mc1r-dependent genes in the skins of neonatal mice compared with melanocytes.
(Download the .jpeg file here - 876KB)
Table S1. ANOVA-selected skin layer genes.
(Download the Excel file here - 3MB)
Table S2. Skin layer gene expression levels relative to melan-a cells.
(Download the Excel file here - 2MB)
Melanoma metastasis gene expression profiles
Supplementary material published to:
Hoek, Keith S. et al.: Metastatic potential of melanomas defined by specific gene expression profiles with no BRAF signature. Pigment Cell Research 19 (4), 290-302.
Appendix:
This file includes all of the supplementary material data referenced in the text. These include sample BRAF/NRAS mutation status and cohort distributions (1), genes with cohort-specific expression (2a), genes with co-regulated expression patterns (2b), data and literature correlations (3a, 3b), and the primer sequences and conditions for RT-PCR analyses (4).
